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Journal: Bioactive Materials
Article Title: Bioengineered titanium implants functionalized with aptamer-valproic acid conjugates orchestrate macrophage programming and mesenchymal stem cell homing for improved osseointegration
doi: 10.1016/j.bioactmat.2026.05.055
Figure Lengend Snippet: A) Live/Dead staining of RAW 264.7 macrophages cultured on different material surfaces for 72 h, where dead cells were stained red and live cells were stained green. B) Cytoskeleton staining morphology of RAW 264.7 grown on different material surfaces for 72 h. C, D, F) Fluorescence images and flow cytometry analysis of intracellular ROS in RAW 264.7 cells with DCFH-DA probe. E) Proliferation of RAW 264.7 macrophages on different material surfaces assessed by CCK-8 assay. n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. Created in BioRender.
Article Snippet: The
Techniques: Staining, Cell Culture, Fluorescence, Flow Cytometry, CCK-8 Assay
Journal: Bioactive Materials
Article Title: Bioengineered titanium implants functionalized with aptamer-valproic acid conjugates orchestrate macrophage programming and mesenchymal stem cell homing for improved osseointegration
doi: 10.1016/j.bioactmat.2026.05.055
Figure Lengend Snippet: A, F) IF staining of iNOS and CD206 in RAW 264.7 macrophages. B-E) Secretion of inflammation-related proteins in RAW 264.7 macrophages. G-J) Relative mRNA expression of inflammation-related genes in RAW 264.7 macrophages. n = 3. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
Article Snippet: The
Techniques: Staining, Expressing
Journal: Materials Today Bio
Article Title: Nano-bionic bacteria armed adoptive macrophage therapy against bladder cancer
doi: 10.1016/j.mtbio.2026.103130
Figure Lengend Snippet: Preparation and characterization of BM@MnO 2 @AB680 nanoparticles. (a) The TEM image of SiO 2 @MnO 2 nanoparticles. (b) The TEM image of hollow MnO 2 (MnO 2 ) nanoparticles. (c) The TEM image of hollow MnO 2 loaded with AB680 (MnO 2 @AB680) nanoparticles. (d) The TEM image of bacterial membrane (BM)-coated hollow MnO 2 loaded with AB680 (BM@MnO 2 @AB680) nanoparticles. (e) The particle sizes of different MnO 2 based nanoparticles. (f) The zeta potentials of BM and different nanoparticles. (g) The PDI of different MnO 2 based nanoparticles. (h) The particle size stability of BM@MnO 2 @AB680 nanoparticles. (i) The zeta potential stability of BM@MnO 2 @AB680 nanoparticles. (j) The PDI stability of BM@MnO 2 @AB680 nanoparticles. (k) Relative oxygen level in different groups within PBS added with H 2 O 2 . (l) CLSM images of RAW264.7 cells incubated with Cy5.5 labeled MnO 2 @AB680 (left) and BM@MnO 2 @AB680 (right) nanoparticles. Scale bar: 100 μm. (m) Mean fluorescence intensity (MFI) of RAW264.7 cells after incubation with MnO 2 @AB680 (Mac@AMn) and BM@MnO 2 @AB680 (Mac@ABMn) nanoparticles. (n) MFI of RAW264.7 cells after incubation with BM@MnO 2 @AB680 with different time.
Article Snippet:
Techniques: Membrane, Zeta Potential Analyzer, Incubation, Labeling, Fluorescence